The Bionutrition Core Laboratory is committed to assisting investigators pursue their research goals. Our primary tools of bioanalytical service include LC-MS/(U)HPLC and spectrophotometry, and custom services for dietary interventions in rodents, gene expression studies, and other biomedical research needs are available upon inquiry.
LC-MS / (U)HPLC
LC-MS and (U)HPLC-UV/Vis, -FL, and -ECD detection is available to quantify analytes of biological interest. The analysis of many phytochemicals, antioxidants, biomarkers of oxidative stress and gastrointestinal permeability are well-established. Custom analyses also can be developed.
SPECTROPHOTOMETRY
Spectrophotometric techniques using UV, Vis, or FL-based detection are available to assess biomarkers of metabolic health, enzyme activities, nitric oxide metabolism, and biomarkers of inflammation. Custom analyses can be developed.
Services Available (Approved FY24)
| Routine Services | Price ($/sample) |
|---|---|
| Plasma Glucose (UV Spectrophotometry) | 13 |
| Plasma Triglyceride (UV Spectrophotometry) | 13 |
| Plasma Total Cholesterol (UV Spectrophotometry) | 13 |
| Plasma HDL-C (UV Spectrophotometry) | 13 |
| Plasma Arachidonic Acid (LC-MS) | 52 |
| Plasma Vitamin E (HPLC-ECD or HPLC-FL) | 52 |
| Plasma Vitamin E (LC-MS) | 97 |
| Plasma Homocysteine (HPLC-FL) | 32 |
| Plasma Arginine, ADMA, SDMA, and Homoarginine (HPLC-FL) | 71 |
| Plasma TNF-α or IL-6 (ELISA, price per increment of 80 samples) | 3040 |
| Plasma C-Reactive Protein (ELISA, price per increment of 80 samples) | 5200 |
| Plasma Oxidized LDL (ELISA, price per increment of 80 samples) | 6880 |
| Custom UV/Vis/FL Spectrometry Analytical Service | Price ($/sample) |
|---|---|
| Clinical Chemistry – Level 1 | 7 |
| Clinical Chemistry – Level 2 | 13 |
| Clinical Chemistry – Level 3 | 24 |
| Clinical Chemistry – Level 4 | 31 |
| Clinical Chemistry – Level 5 | 39 |
| Clinical Chemistry – Level 6 | 45 |
| Note: Fee structure for clinical chemistry analysis is based on complexity of biospecimen preparation. Factors considered include: type of extraction required, requirements for specialized chemicals/reagents and/or commercial kits, and type of instrumentation used for analysis (e.g., spectrophotometer, fluorometer). Inquire within to discuss your project | |
| Custom (U)HPLC/LC-MS Service | Price ($/sample) |
|---|---|
| HPLC – Level 1 | 13 |
| HPLC – Level 2 | 20 |
| HPLC – Level 3 | 30 |
| HPLC – Level 4 | 41 |
| HPLC – Level 5 | 52 |
| HPLC – Level 6 | 71 |
| HPLC – Level 7 | 84 |
| HPLC – Level 8 | 97 |
| HPLC – Level 9 | 119 |
| Note: Fee structure for (U)HPLC/LC-MS analysis is based on complexity of biospecimen preparation and sophistication of analytical instrumentation needed. Factors considered include: sample homogenization, liquid-liquid or solid-phase extraction, requirements for specialized chemicals/reagents, advanced filtration requirements, separation column required, sample derivatization, and type of instrumentation (e.g., conventional HPLC, UPLC, or LC-MS) and detection mode (UV/Vis, FL, MS, Electrochemical) needed to support analysis. Inquire within to discuss your project | |
| Other Available Services | Fee ($) |
|---|---|
| LC-MS Equipment Rental (per hour) | 18 |
| Method Development Fee (per hour) | 150 |
| Individual Training Session (per hour) | 25 |
| Return Shipping of Biospecimens (per box) | 80 |